Technical Data
P9009-56G
Prolactin Receptor (PRLR, PRL-R, PRL Receptor)
Description:
Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription. Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40kD to 100kD. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Applications:
Suitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry: 5ug/ml
Immunohistochemistry: Frozen and paraffin sections
Western Blot: 1ug/ml, detects bands of ~40 and ~100kD representing both the short and the long forms of the prolactin receptor.
Immunofluorescence: 1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D695Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Purified rat liver prolactin receptor.
Purity:
Purified
Form
Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes the extracellular domain of rat prolactin receptor. Does not inhibit the interaction of prolactin with the receptor. Species Crossreactivity: human, rabbit and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
11. Characterization and applications of monoclonal antibodies to the prolactin receptor: H. Okamura, et al.; Endocrinology 124, 2499 (1989) 2. The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation: M. Royster, et al.; Endocrinology 136, 3892 (1995) 3. Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation: M. Perrot-Applanat, et al.; J. Cell Sci. 110, 1123 (1997) 4. The endogenous cannabinoid anandamide inhibits human breast cancer cell proliferation: L. De Petrocellis, et al.; PNAS 95, 8375 (1998)