Technical Data
P9052-29
Proprotein Convertase 9 (PC9, PCSK9, Neural Apoptosis-Regulated Convertase 1, NARC1)
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Technical Data |
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P9052-29 |
Proprotein Convertase 9 (PC9, PCSK9, Neural Apoptosis-Regulated Convertase 1, NARC1) |
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Description: Proprotein Convertase 9 encoded by the PCSK9 gene, (PC9 or NARC1 for neural apoptosis-regulated convertase-1), regulates cholesterol trafficking by controlling the plasma levels of LDL receptors. Different mutations in PCSK9 are associated with either elevated or reduced plasma levels of LDL-cholesterol. Human PCSK9 consists of a pro domain (aa29-152) and a mature chain (aa153-692). Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse PC9 is ~50ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold. Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human and mouse PC9 from conditioned cell metdium. Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse PC9 is ~5ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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