Technical Data
Proprotein Convertase 9 (PC9, PCSK9, Neural Apoptosis-Regulated Convertase 1, NARC1)
Proprotein Convertase 9 encoded by the PCSK9 gene, (PC9 or NARC1 for neural apoptosis-regulated convertase-1), regulates cholesterol trafficking by controlling the plasma levels of LDL receptors. Different mutations in PCSK9 are associated with either elevated or reduced plasma levels of LDL-cholesterol. Human PCSK9 consists of a pro domain (aa29-152) and a mature chain (aa153-692).

Suitable for use in Western Blot, Immunoprecipitation and Direct ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml. Using recombinant human propprotein convertase 9 under non-reducing conditions.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human and mouse PC9 from conditioned cell medium.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG17H232Affinity Purified
100ug-20CBlue IceMouseRat
~0.5mg/ml (after reconstitution)
Recombinant protein corresponding to aa35-694 from mouse Proprotein Convertase 9 expressed in NSO cells (Q80W65). Species Sequence Homology: Rat/mouse-77%, chimpanzee-99%.
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes the mature form of mouse Proprotein Convertase 9 (PC9). Species Crossreactivity: human. Does not crossreact with recombinant human PC1 or PC7.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.