Technical Data
P9052-29
Proprotein Convertase 9 (PC9, PCSK9, Neural Apoptosis-Regulated Convertase 1, NARC1)
Description:
Proprotein Convertase 9 encoded by the PCSK9 gene, (PC9 or NARC1 for neural apoptosis-regulated convertase-1), regulates cholesterol trafficking by controlling the plasma levels of LDL receptors. Different mutations in PCSK9 are associated with either elevated or reduced plasma levels of LDL-cholesterol. Human PCSK9 consists of a pro domain (aa29-152) and a mature chain (aa153-692).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse PC9 is ~50ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human and mouse PC9 from conditioned cell metdium.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse PC9 is ~5ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17H232Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa35-694 from mouse Proprotein Convertase 9 expressed in NSO cells (Q80W65). Species Sequence Homology: Rat/mouse-77%, chimpanzee-99%.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes the mature form of mouse Proprotein Convertase 9 (PC9). Species Crossreactivity: Human. Does not crossreact with recombinant human PC1 or PC7.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.