Technical Data
P9052-40
Proprotein Convertase Subtilisin Kexin 9 (PCSK9, NARC-1)
Description:
Proprotein convertase subtilisin kexin 9 (PCSK9) is a member of the subtilisin serine protease family with an important role in lipoprotein metabolism. Mutation in the PCSK9 gene is associated with autosomal dominant hypercholesterolemia which is characterized by an increase in low density lipoprotein (LDL) cholesterol levels. PCSK9 overexpression in wild-type mice doubles the plasma total cholesterol, possibly through acceleration of the degradation of the LDL receptor. PCSK9 mRNA is detected in various tissues such as liver, kidney, lung, spleen, jejunum, ileum, colon, and muscle with the highest expression in the liver. Human PCSK9 precursor is 692 amino acid in length with an estimated molecular weight of 74kD. This proprotein is self-cleaved to form a mature protein at around 63kD in the golgi.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100 (3ug/ml)
Immunofluorescence (IC): 1:25 (16ug/ml)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide corresponding to aa490-502, SRSGKRRGERMEA of human PCSK9.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human PCSK9. Detects mainly the mature form of the protein ranging from 62-66kD in tissues and cells such as liver, kidney and colon cancer cells. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Maxwell, K.N.et al., Proc. Natl. Acad. Sci. USA 102(6): 2069-2074 (2005). 2. Abifadel, M., et al., Nature Genet. 34(2): 154-156 (2003). 3. Maxwell, K.N. & Breslow, J.L., Proc. Natl. Acad. Sci. USA 101(18): 7100-7105 (2004). 4. Seidah, N.G., et al., Proc. Natl. Acad. Sci. USA 100(3): 928-933 (2003). 5. Maxwell, K.N. & Breslow, J.L., Curr. Opin. Lipidol. 16: 167-172 (2005).