Technical Data
P9052-40D
PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9, Neural Apoptosis-regulated Convertase 1, NARC-1, Proprotein Convertase 9, PC9, Subtilisin/Kexin-like Protease PC9, NARC1, PSEC0052)
Description:
PCSK9 is a serine protease of the protein convertase family having profound effects on plasma low-density lipoprotein (LDL)-cholesterol (LDL-C) levels through its ability to mediate LDL receptor (LDLR) protein degradation. The protein slows down the uptake of cholesterol by degrading LDLR after they have internalized LDL and thus plays a significant role in cholesterol homeostasis. PCSK9 also potentially averts excessive cholesterol accumulation within the cell by preventing the recycling of LDLRs to the cell surface. Structurally, the protein includes a signal peptide, followed by a prodomain, a subtilisin-like catalytic domain, and a variable C-terminal domain. The prodomain serves a dual role as a chaperone for folding and as an inhibitor of catalytic activity. Human PCSK9 gene is localized in the chromosomal region 1p32.3. Several mutations (p.S127R, p.F216L, p.D374Y) of this gene have been reported to cause hypercholesterolemia.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabigGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CRSRHLAQASQELQ, from the C-terminus of the protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes PCSK9. Species Crossreactivity: bison, broad, c elegans, human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.