Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Flow Cytometry: Cells were treated with phospholipase C before staining with the antibody.
Immunohistochemistry: Fresh frozen tissues.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Whole live cells of six human prostatic carcinoma cell lines, ALVA31, DU145, JCA1, LNCaP, ND1 and PC3.|
|Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.|
|Recognizes the cell surface transmembrane glycoprotein of human prostate epithelial cells at ~110kD. Reacts with both benign and malignant cells. Reacts with DU145, ND1, PC3 and ALV31 cell lines. No reactivity with LNCaP and JCA1 cell lines.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Rokhlin, O.W., et al., Cancer Letters 131(2): 129-136 (1998). 2. Rokhlin, O.W., et al., Cancer Research 57: 1758-1768 (1997).|