Technical Data
P9054-03
Prostate Cell Surface Antigen
Description:
Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Cells were treated with phospholipase C before staining with the antibody.
Immunohistochemistry: Fresh frozen tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q458Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Whole live cells of six human prostatic carcinoma cell lines, ALVA31, DU145, JCA1, LNCaP, ND1 and PC3.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes the cell surface transmembrane glycoprotein of human prostate epithelial cells at ~110kD. Reacts with both benign and malignant cells. Reacts with DU145, ND1, PC3 and ALV31 cell lines. No reactivity with LNCaP and JCA1 cell lines.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rokhlin, O.W., et al., Cancer Letters 131(2): 129-136 (1998). 2. Rokhlin, O.W., et al., Cancer Research 57: 1758-1768 (1997).