Technical Data
P9054-67L
ACPP (Prostatic Acid Phosphatase, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, Thiamine Monophosphatase, TMPase)
Description:
Prostate specific acid phosphatase (PSAP) is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase. This antibody does not inhibit the enzymatic activity of PSAP. It reacts with nonneoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no cross reaction with other phosphatases.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-YGIHKQKEKSRLQ, from human ACPP, at the internal region of the protein (NP_001090.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human ACPP. Species sequence homology: Bovine, canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Quintero IB, Araujo CL, Pulkka AE, Wirkkala RS, Herrala AM, Eskelinen EL, Jokitalo E, Hellström PA, Tuominen HJ, Hirvikoski PP, Vihko PT. Prostatic acid phosphatase is not a prostate specific target. Cancer research 2007 Jul 67(14):6549-54.