Technical Data
P9054-78B
Prostatic Binding Protein (PBP, Hippocampal Cholinergic Neurostimulating Peptide, HCNP, HCNPpp, Neuropolypeptide h3, Phosphatidylethanolamine Binding Protein, PEBP, Phosphatidylethanolamine Binding Protein 1, PEBP1, PEBP-1, Raf Kinase Inhibitory Protein,
Description:
PBP binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. It is a serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (by similarity).
PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. It seems to be mediated by a specific receptor (by similarity).

Positive Control: A549, HepG2 and Human Liver lysate.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: DYVPKLYEQLSGK representing the C-Terminus of the human protein (residues 174-186), according to NP_002558.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Specificity:
Species Crossreactivity: Human. Expected to crossreact with Mouse and Rat due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Yeung K et al. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature 401:173-7 (1999).
PUBMED:10490027