		Technical Data
P9054-78D	Prostatic Binding Protein (PBP, Hippocampal Cholinergic Neurostimulating Peptide, HCNP, HCNPpp, Neuropolypeptide h3, Phosphatidylethanolamine Binding Protein, PEBP, Phosphatidylethanolamine Binding Protein 1, PEBP1, PEBP-1, Raf Kinase Inhibitory Protein,

Description:
Phosphatidylethanolamine binding protein (PEBP), also known as Raf kinase inhibitory protein (RKIP) is a highly conserved multifunctional protein that modulates the Raf/MAPK signaling cascade and has been implicated as a suppressor of metastatic cancer (Trakul N et al., 2005). PEBP has been shown to not only inhibit cell proliferation but also to induce differentiation of human keratinocytes (Yamazaki et al., 2004). Dysregulation of calpain activity results in cellular degeneration in conditions such as Alzheimer’s disease and recently PEBP has been identified as a potential in vivo calpain substrate (Chen at al., 2006)

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Rat	Rabbit

Concentration:

As Reported

Immunogen:

Synthetic peptide corresponding to native PEBP protein purified from rat.

Purity:

Serum

Form

Supplied as a liquid.

Specificity:

Recognizes rat Phosphatidylethanolamine binding protein at ~22k.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Trakul N, Menard RE, Schade GR, Qian Z, Rosner MR (2005) Raf kinase inhibitory protein regulates Raf-1 but not B-Raf kinase activation. J. Biol. Chem. 280(26): 24931-40. Yamazaki T, Nakano H, Hayakari M, Tanaka M, Mayama J, Tsuchida S (2004) Differentiation induction of human keratinocytes by phosphatidylethanolamine binding protein. J. Biol. Chem. 279(31):32191-95. Chen Q, Wang S, Thompson SN, Hall ED, Guttmann RP (2006) Identification and characterization of PEBP as a calpain substrate. J Neurochem. 99(4): 1133-41.