Technical Data
Prostatic Binding Protein (PBP, Hippocampal Cholinergic Neurostimulating Peptide, HCNP, HCNPpp, Neuropolypeptide h3, Phosphatidylethanolamine Binding Protein, PEBP, Phosphatidylethanolamine Binding Protein 1, PEBP1, PEBP-1, Raf Kinase Inhibitory Protein,
Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:16,000
Western Blot: 0.01-0.03ug/ml ~22kD band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 21.1kD according to NP_002558.1). Reported ~22kD in Rat Hippocampus.
Immunohistochemistry: 1-2ug/ml In paraffin embedded Human Brain (Cortex) shows staining near sectect nuclei.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-DYVPKLYEQLSGK, from the C Terminus of human PEBP1 (NP_002558.1).
Purified by peptide affinity chromatography.
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Recognizes human PEBP1. Species sequence homology: mouse, rat, canine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Yeung K, Seitz T, Li S, Janosch P, McFerran B, Kaiser C, Fee F, Katsanakis KD, Rose DW, Mischak H, Sedivy JM, Kolch W. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP.
Nature. 1999 Sep 9;401(6749):173-7.