		Technical Data
P9054-85	Prosurfactant Protein B, CT (proSP-B)

Description:
Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. It is composed primarily of phospholipids (phosphatidylcholine and phosphatidylglycerol) and lesser amounts of cholesterol and surfactant-associated protein. Prosurfactant Protein B is a precursor of a very hydrophobic protein of the surfactant.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:2000-1:4000 on human tissue with and without antigen retrieval and 1:1000-1:2000 on adult mouse lung or 1:500 on fetal mouse lung.
Western Blot: 1:1000-1:5000
ELISA: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not Determined

Immunogen:

Recombinant peptide containing 102 residues of the C-terminal fragment of the human SP-B proprotein.

Purity:

Serum

Form

Supplied as a liquid in PBS. No preservatives added.

Specificity:

Recognizes human Prosurfactant Protein B, CT. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Lin, S., et al., J. Biol. Chem. (1996) 271:19689–19695. Lin, S., et al., Biochim. Biophys. Acta (1996) 1312:177–185. Zhou, L., et al., Dev. Biol. (1996) 175:227–238. Zhou, L., et al., J. Histochem.Cytochem. (1996) 44:1183–1193. Jain-Vora, S., et al., Am. J. Respir. Cell Mol. Biol. (1997) 17:1–11. Zsengeller, Z.K., et al., Hum. Gene Ther. (1997) 8:1331–1344. Tokieda, K., et al., Am. J. Respir. Cell and Mol. Biol. (1999) 21:463–472. Nogee, L.M., et al., Am. J. Respir. Crit. Care Med. (2000) 161:973–981.