Technical Data
P9054-85
Prosurfactant Protein B, CT (proSP-B)
Description:
Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. It is composed primarily of phospholipids (phosphatidylcholine and phosphatidylglycerol) and lesser amounts of cholesterol and surfactant-associated protein. Prosurfactant Protein B is a precursor of a very hydrophobic protein of the surfactant.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:2000-1:4000 on human tissue with and without antigen retrieval and 1:1000-1:2000 on adult mouse lung or 1:500 on fetal mouse lung.
Western Blot: 1:1000-1:5000
ELISA: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Recombinant peptide containing 102 residues of the C-terminal fragment of the human SP-B proprotein.
Purity:
Serum
Form
Supplied as a liquid in PBS. No preservatives added.
Specificity:
Recognizes human Prosurfactant Protein B, CT. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lin, S., et al., J. Biol. Chem. (1996) 271:1968919695. Lin, S., et al., Biochim. Biophys. Acta (1996) 1312:177185. Zhou, L., et al., Dev. Biol. (1996) 175:227238. Zhou, L., et al., J. Histochem.Cytochem. (1996) 44:11831193. Jain-Vora, S., et al., Am. J. Respir. Cell Mol. Biol. (1997) 17:111. Zsengeller, Z.K., et al., Hum. Gene Ther. (1997) 8:13311344. Tokieda, K., et al., Am. J. Respir. Cell and Mol. Biol. (1999) 21:463472. Nogee, L.M., et al., Am. J. Respir. Crit. Care Med. (2000) 161:973981.