Technical Data
P9054-86
Prosurfactant Protein B (proSP-B)
Description:
Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. It is composed primarily of phospholipids (phosphatidylcholine and phosphatidylglycerol) and lesser amounts of cholesterol and surfactant-associated protein. Prosurfactant Protein B is a precursor of a very hydrophobic protein of the surfactant.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:4000-1:8000 (Human tissue with and without antigen retrieval)
Immunohistochemistry: 1:1000-1:2000 (Adult and fetal mouse lung)
Western Blot: 1:1000-1:5000
ELISA: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Recombinant peptide corresponding to full length (381aa) human SP-B (pre)proprotein.
Purity:
Serum
Form
Supplied as a liquid. No preservative added.
Specificity:
Recognizes human proSP-B (detects both the N-terminal and C-terminal propeptides). Does not crossreact with mature SP-B. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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