Technical Data
P9076-14C
PSMB4 (Proteasome Subunit beta Type-4, Proteasome beta Chain, Macropain beta Chain, Multicatalytic Endopeptidase Complex beta Chain, Proteasome Chain 3, HsN3, 26kD Prosomal Protein, PROS-26, HsBPROS26, PROS26) (Azide Free)
Description:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq]

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 3ng/ml-1ug/ml
Immunohistochemistry (paraffin): 1.5ug/ml
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k11C602Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant protein corresponding to a portion of amino acids within aa1-265 of human PSMB4 with GST tag. MW of GST tag = 26kD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (azide free).
Specificity:
Recognizes human PSMB4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.