Technical Data
P9078-38E
PSMB1, CT (Proteasome gamma Chain, Multicatalytic Endopeptidase Complex Subunit C5, Macropain Subunit C5, Proteasome Component C5, Proteasome Subunit beta Type-1, PSC5)
Description:
PSMB1 is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa212-241 from the C-terminal region of human PSMB1.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human PSMB1. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Okumura,K.,et.al., Genomics 27(2), 377-379 (1995).