Technical Data
Purification Media, Avidin Beads, BioSeparation™
Molecular Biology Storage: 4°CShipping: RT
(Biotin-Capture) Avidin beads are useful carriers for biotinylated molecules, in various applications such as the construction of affinity columns or for conducting immuno-precipitations (IP). Biotin-Capture Beads contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical Stability. Modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications.

Biotin-Capture Beads Specifications:
Matrix: Sepharose CL-4B
Coupling method: TargetLock
Type of Avidin bound to beads: Modified Avidin
Binding capacity: 2-4mg biotinilated BSA per ml of beads
Bead size: 40-165um
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3MPa, 3 bar
Max. flow rates: 4ml/min/cm2
Recommended flow rate: 1-2ml/min/cm2
Stability of the matrix: pH 2-11.
Storage: 4°C in PBS pH 7.4 added with sodium azide 0.05% (w/v) as a preservative.

Store gel refrigerated in phosphate buffered saline (PBS) or other ligand-compatible
buffer added with 0.1% azide (w/v) as a preservative until use.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.