Technical Data
P9100-67
Purification Media, BioSeparation™ Affinity, Glutathione
5ml
10ml
10ml
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Technical Data |
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P9100-67 |
Purification Media, BioSeparation™ Affinity, Glutathione |
5ml 10ml |
| BioSeparation? | Storage: 4°CShipping: RT |
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Glutathione Beads are used for the purification of glutathione S- transferase (GST) tagged proteins, produced using various commercial expression vectors. GST-fused proteins can be purified from bacterial lysates by one-step affinity purification. Glutathione Beads is state of the art affinity-resin product that consists of glutathione attached through sulfur to an epoxy-activated 4% cross-linked beaded agarose. The use of the Glutathione Beads enables the purification of various GST-fused proteins, while maintaining mild, non-denaturing conditions throughout the purification process. Glutathione Beads characteristics: Matrix: Sepharose CL-4B Activation method: Oxiran Binding capacity: 4-8mg/ml recombinant GST Bead size: 40-165um Bead structure: Highly cross-linked spherical agarose, 4% Max back pressure: 0.3MPa, 3bar Max. flow rate: 4ml/min/cm2 Recommended flow rate: 1-2ml/min/cm2 Stability of the matrix: pH 4-10 Storage and Stability: Store at 4°C in PBS pH 7.4 with with sodium azide 0.1% (w/v) added as preservative. |
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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