Technical Data
P9100-70
Purification Media, BioSeparation™ Affinity, Protein A, Recombinant
10mg
50mg
Molecular Biology Storage: 4°CShipping: RT
Recombinant Protein-A used also in Protein A beads (P9100-75) is a genetically engineered form of Protein-A derived from Staphylococcus aureus. Non-essential regions have been modified while leaving the IgG binding sites intact. Protein A is been used extensively used for the isolation of IgG from several species of mammals. The affinity of Protein A to IgG's is different between species and IgG subclasses within a given species. A major known limitation of protein A is its weak binding to Mouse IgG1– a major immunoglobulin subclass in Mice. Despite its variable binding characteristics, Protein A is a popular choice for both antibodies binding in various assays and when coupled to beads, for Immunoprecipitation of -antigen complexes. Protein A binds specifically the Fc region of immunoglobulins leaving the Fab region free for binding the antigen. Hence, Protein A is extremely useful for isolating of immune complexes.

Protein A Beads characteristics:
Source: Recombinant protein
Molecular Weight: 23.3kD expressed in E. coli
Purity: 90% by SDS-PAGE.
Spectroscopic analysis: OD280nm/250nm=2.51
Endotoxin: 0.5EU/mg Recombinant Protein A (LAL)
Composition: Lyophilized white Powder. 10.9mg Recombinant Protein A, 0.82 mg
phosphate buffer salts.

Storage before Reconstitution: Store at 4°C. Product is stable under these conditions for
one year.

Storage after Reconstitution: Store at 4°C in PBS pH 7.4 added with sodium azide 0.1% (w/v) as a preservative. Product is stable under these conditions for 1 month. Alternatively, prepare small aliquots and store at -20°C for up to two years. Avoid repeated freezing and thawing cycles.

Storage and Stability:
Stable at RT for 1 week
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.