Technical Data
Purification Media, BioSeparation™ Affinity, Protein A Beads (Agarose)
Molecular Biology Storage: 4°CShipping: RT
The origin of Protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from goat, rabbit, mouse and human.
Protein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to agrose beads using TargetLock chemistry, in order to create a more stable product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins.

Protein A beads are being routinely used for the isolation of IgG from several species of mammals. The binding of Protein A to IgGs vary between animal species and between IgG subclasses within the same species. Major limitation of protein A lies with the weak binding it
presents towards mouse IgG1, a common IgG subclass. Despite that, Protein A possesses useful
properties that make it a popular choice for the isolation of most types of IgGs. Protein A binds IgGs through the Fc region of the molecules leaving the Fab region available for binding the antigen. Protein A Beads are commomnly used for antibodies purification and for the isolation of immune complexes by Immunoprecipitation.

Protein A Beads Specifications:

Sepharose CL-4B

Coupling method: TargetLock.

Protein A Density:
2-4mg/ml of resin

Binding Capacity:
~4-8mg rabbit IgG per ml of resin

Mean bead size:
40 -165um

Bead structure:
Highly cross-linked spherical agarose, 4%

Max back pressure:
0.3 MPa, 3 bar

Max. flow rate:
4 ml/min/cm2

Recommended flow rate:
1-3 ml/min/cm2

Stability of the matrix:
pH 2-11

Storage and Stability:
May be stored at 4°C. Do not freeze. Stable for at least 6 months.
Form: Supplied as a suspension in PBS, pH 7.4, 0.1% sodium azide.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.