Technical Data
P9100-85
Purification Media, BioSeparation™ Affinity, Protein G (Agarose)
1ml
5ml
10ml
BioSeparation? Storage: 4°CShipping: RT
Protein G originated from Streptococci is a monomeric protein with a molecular weight of about
50,000 kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of
immunoglobulins from Mouse, Rabbit, Goat and Human. Due to this binding ability, Protein G is
commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads, using TargetLock chemistry, in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).

Matrix:
SepharoseTM CL-4B
Coupling method: TargetLock
Protein G Density: 3-4mg/ml of gel
Binding Capacity:
~4-8mg rabbit IgG per ml of beads
Mean bead size:
40-165um
Bead structure:
Highly cross-linked spherical agarose, 4%
Max back pressure:
0.3MPa, 3 bar
Max. flow rates: 4ml/min/cm2
Recommended flow rate: 1-3 ml/min/cm2
Stability of the matrix:
pH 2-11.
Storage:
Store gel refrigerated at 4°C in PBS pH 7.4 containing 0.1% sodium azide as a preservative
 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.