Technical Data
Purification Media, BioSeparation™ Affinity, Protein G (Agarose)
BioSeparation? Storage: 4°CShipping: RT
Protein G originated from Streptococci is a monomeric protein with a molecular weight of about 50,000kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of immunoglobulins from mouse, rabbit, goat and human. Due to this binding ability, Protein G is commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).

SepharoseTM CL-4B

Coupling Method: TargetLock

Protein G Density: 3-4mg/ml of gel

Binding Capacity:
~4-8mg rabbit IgG per ml of beads

Mean Bead Size:

Bead Structure:
Highly cross-linked spherical agarose, 4%

Max Back Pressure:
0.3MPa, 3 bar

Max. Flow Rates: 4ml/min/cm2

Recommended Flow Rate: 1-3 ml/min/cm2

Stability of the Matrix:
pH 2-11.

Store gel refrigerated at 4°C in PBS pH 7.4 containing 0.1% sodium azide as a preservative, or PBS pH 7.4 containing 20% ethanol.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.