Technical Data
P9102-27
Protein C (Coagulation Factor XIV)
Description:
Coagulation factor XIV, also known as Protein C, has anti-coagulation activity by degrading factors VIIIa and Va, which are required for thrombin activation. Protein C deficiency results in hereditary thrombophilia, and a severe recessive form may result in massive thrombosis fatal to patient. The deduced amino acid sequence of human protein C predicts a signal peptide (aa 1-32), a propeptide (aa 33-42), and a mature chain (aa 43-461), which can be converted into two disulfide-linked chains (light: aa 43- 199 and heavy: aa 200-461). The amino acid sequence of human protein C is 99.8%, 80%, 70%, 69% and 54% identical to that of chimpanzee, canine, rat, mouse and chicken.

Applications:
Suitable for use in Western Blot, Immunopreciptiation and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunopreciptiation: 25ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17H185Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
NS0-derived recombinant human Coagulation Factor XIV (aa43-461) Accession # NP_000303
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes human Coagulation Factor XIV.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.