		Technical Data
P9102-29	Protein C

Description:
Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62kD proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55kD and the normal concentrations in human plasma is approximately 1-3ng/ml because of the very fast turnover, the proenzyme concentration is ~3ug/ml.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000. Strong reaction is seen in capture ELISA in combination with a biotin labeled antigen against Protein C only when using a tris-buffer containing 2.5mM CaCl2
Western Blot: After SDS-PAGE, reacts with protein C in non-reduced form only.
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63.Ag8.653 myeloma cells with spleen cells of female CF1xBalb/c mice.

Culture Medium:
RPMI 1640 with 10% fetal calf serum

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	6F182	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Protein C isolated from human plasma.

Purity:

Purified by Protein A/G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Specificity:

Recognizes human Protein C.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.