Technical Data
Protein C
Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62kD proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55kD and the normal concentrations in human plasma is approximately 1-3ng/ml because of the very fast turnover, the proenzyme concentration is ~3ug/ml.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:30,000. Strong reaction is seen in capture ELISA in combination with a biotin labeled antigen against Protein C only when using a tris-buffer containing 2.5mM CaCl2
Western Blot: After SDS-PAGE, reacts with protein C in non-reduced form only.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k6F182Affinity Purified
100ul-20CBlue IceHumanMouse
Protein C isolated from human plasma.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Recognizes human Protein C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.