Technical Data
P9102-29A
Protein C
Description:
Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62kD proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55kD and the normal concentrations in human plasma is approximately 1-3ng/ml because of the very fast turnover, the proenzyme concentration is approximately 3ug/ml.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Female CF1x Balb/c mice.

Culture Medium:
RPMI 1640 with 10% fetal calf serum.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k6F183Affinity Purified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Protein C isolated from human plasma.
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Before the addition of glycerol to 40%.
Specificity:
Recognizes human Protein C. Epitope specificity differs from that of clone 6F182. Strong reaction is seen in sandwich ELISA in combination with a biotin labeled antigen against Protein C, or in combination with at polyclonal antibody against human Protein C. In Western Blot after SDS-PAGE reacts with protein C in non-reduced form only.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.