Technical Data
P9102-35A
Protein C
Description:
Protein C (PC) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of PC in plasma is ~4ug/ml (~60nM). A deficiency of Protein C (quantitative or qualitative) is a risk factor for vascular thrombosis. Protein C is expressed as a two-chain molecule with a molecular weight of 62 kDa. The light chain (21kDa) of PC consists of two EGF-like domains and an amino-terminal domain containing one hydroxyaspartic acid and 11g-carboxyglutamic acid (gla) residues. These residues allow PC to bind to membranes that contain acidic phospholipids in a calcium dependent manner. The heavy chain of PC (41kDa) consists of the catalytic domain and an activation peptide. Activation of Protein C results from cleavage at residue Arg12 in the heavy chain by a complex of thrombin and a cell surface cofactor thrombomodulin. The activation of PC is associated with the release of a small activation peptide (2-3 kDa, called Protein C peptide, or PCP) from the N-terminal of the heavy chain. Activated Protein C (APC) is a serine protease with anticoagulant activity. APC, in complex with a phospholipid membrane, calcium and the Protein S cofactor, exhibits anticoagulant activity through the proteolytic inactivation of coagulation cofactors Va and VIIIa. The primary inhibitor of APC activity in plasma is Protein C Inhibitor (PCI, also called Plasminogen Activator Inhibitor-3, PAI-3) and to a lesser extent by alpha A1antitrypsin and alpha 2macroglobulin. The inhibitory activity of PCI is stimulated approximately 10 fold by heparin.

Application:
Suitable for use in ELISA and rocket immunoelectrophoresis (RIEP) for the measurement of Protein C antigen in plasma samples. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
~5mg/ml
Immunogen:
Protein C (PC) purified from human plasma.
Purity:
Purified
Form
Supplied as a liquid in 10mM HEPES, pH7.4, 0.15M sodium chloride, 50% glycerol.
Specificity:
Recognizes human Protein C as demonstrated by immunoelectrophoresis and ELISA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Broze GJ, Miletich JP; Biochemistry and Physiology of Protein C, Protein S and Thrombomodulin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 259-276, J.B. Lippincott Co., Philadelphia PA, USA, 1994.2. Esmon CT, Esmon NL, Le Bonniec B, Johnson AE; Protein C Activation; Methods in Enzymology 222, pp 359-385, 1993. 3. Heeb MJ, Mosher D, Griffin JH; Activation and Complexation of Protein C and Cleavage and Decrease of Protein S in Plasma of Patients With Intravascular Coagulation; Blood 73, pp 455-461, 1989.