Technical Data
P9102-39A
Activated Protein C (APC)
Description:
Protein C (PC) is an important regulator of the blood coagulation system. The anticoagulant PC is converted to activated protein C (APC) by the thrombomodulin (TM)–thrombin complex on the phospholipid surface of endothelial cells, monocytes, and platelets. This process is enhanced in the presence of the endothelial protein C receptor (EPCR). In addition to its regulatory function in the blood coagulation system, studies indicate important roles for APC in inflammatory processes. The administration of APC prevents lethal effects of Escherichia coli–associated sepsis in experimental animal models and appears effective for the treatment of patients with meningococcemia or acquired PC deficiency. Treatment with APC improves the clinical outcome of patients with sepsis. The anti-inflammatory activity of APC depends on its ability to suppress the secretion of cytokines as tumor necrosis factor-alpha by inflammatory cells, the activation and extravasation of leukocytes and the expression and function of adhesion molecules. APC may also suppress the expression of growth factors and members of nuclear factor-kB (NF-kB) family of transcription factors.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:50
Western Blot: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2aPC107Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRat
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes activated human protein C (APC) and lacks reactivity for Protein C (PC). Recognizes the light chain of the APC molecule.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.