Technical Data
P9103-59R2
Protein Kinase C, mu, Protein Kinase D, phosphorylated (Ser916) (PKCm, PKD)
Description:
PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30kD protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:50,000-1:100,000
Immunohistochemistry: 1:100-1:250
Immunoprecipitation: 1:60
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E486Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A phospho-specific peptide corresponding to residues surrounding S916 on human Protein Kinase C.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human Protein Kinase C, mu phosphorylated on Serine 916.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Johannes, F.J. et al. J Biol Chem. 269:6140-6148 (1994). 2. Maeda, Y. et al., EMBO J 20:5982-5990 (2001). 3. Jamora, C., et al., Cell 98:59-68 (1999).