Technical Data
P9104-92D
Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent Activator (PRKRA, PACT, RAX)
Description:
Protein kinase, interferon-inducible double stranded RNA dependent activator (PACT, PRKRA) is a proapoptotic cellular protein activator of PKR, a double-stranded RNA-dependent protein kinase (1). PACT is reported to be stress-activated, and a physiologic activator that couples transmembrane stress signals and protein synthesis (2). Overexpression of PACT in cells causes enhanced sensitivity to stress-induced apoptosis. It is expressed at high levels in colonic epithelial cells, especially as they exit the cell cycle and enter an apoptotic program (3).

Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9E492Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues on the C terminus of human PACT.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human PACT. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Patel RC, et al. EMBO J. 17(15):4379-90, 1998 2. Ito T, et al. J Biol Chem. 274(22):15427-32, 1999
3. Gupta V, et al. Am J Physiol Gastrointest Liver Physiol 283(3):G801-G808, 2002