Technical Data
Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent Activator (PRKRA, PACT, RAX)
Protein kinase, interferon-inducible double stranded RNA dependent activator (PACT, PRKRA) is a proapoptotic cellular protein activator of PKR, a double-stranded RNA-dependent protein kinase (1). PACT is reported to be stress-activated, and a physiologic activator that couples transmembrane stress signals and protein synthesis (2). Overexpression of PACT in cells causes enhanced sensitivity to stress-induced apoptosis. It is expressed at high levels in colonic epithelial cells, especially as they exit the cell cycle and enter an apoptotic program (3).

Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues on the C terminus of human PACT.
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Recognizes human PACT. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Patel RC, et al. EMBO J. 17(15):4379-90, 1998 2. Ito T, et al. J Biol Chem. 274(22):15427-32, 1999
3. Gupta V, et al. Am J Physiol Gastrointest Liver Physiol 283(3):G801-G808, 2002