Technical Data
P9108-06
Protein S
Description:
Protein S is a vitamin K-dependent, potent neutral anticoagulant plasma glycoprotein. Protein S has a molecular weight of approximately 70kD and concists of a single polypeptide chain. The concentration of protein S in human plasma is 25ug/ml, with a half-life of approximately two days. About 40% of Protein S in plasma is in a free form, whereas 60% is complexed with C4b-binding Protein (C4BP). Only the free Protein S functions as a cofactor to antigen presenting cells (APC) in the APC-dependent degradation of Factor Va and Factor VIIIa.
Protein S is thought to function in both the coagulation and complement cascades. It has been suggested that g-carboxyglutamic acid (gla) dependent binding of protein S to negatively charged phospholipids may function to concentrate C4BP at cell surfaces following injury.
The domain structure of Protein S is similar to that of the other vitamin K-dependent coagulation factors with the exception that Protein S does not possess the catalytic triad. Protein S is a single chain protein containing 10 gla residues in the NH2-terminal domain and 4 epidermal growth factor (EGF) domains.

Applications:
Suitable for use ELISA and Immunoelectrophoresis. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~5mg/ml
Immunogen:
Human Protein S (PS) purified from plasma.
Purity:
Purified by caprylic acid and ammonium sulfate precipitations, and solid phase adsorption to remove unwanted crossreactivity.
Form
Supplied in 10mM HEPES, pH7.4, 150mM sodium chloride, 50% glycerol.
Specificity:
Recognizes human Protein S as demonstrated by immunoelectrophoresis and ELISA. Species crossreactivity: porcine and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
2. Bendtzen K et al (2000) Basal og klinisk immunologi. FADLs Forlag A/S, Copenhagen.