Technical Data
Protein S
Protein S is a vitamin K-dependent, potent neutral anticoagulant plasma glycoprotein. Protein S has a molecular weight of approximately 70kD and consists of a single polypeptide chain. The concentration of protein S in human plasma is 25ug/ml, with a half-life of approximately two days. About 40% of protein S in plasma is in a free form, wheraes 60% is complexed with C4b-binding Protein (C4BP). Only the free protein S functions as a cofactor to antigen presenting cells (APC) in the APC-dependent degradation of Factor Va and Factor VIIIa.

Suitable for use in ELISA. Not suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

X63-Ag8.653: Female CF1 Balb/c mice immunized by intraperitoneal injection.

Culture Medium:
RPMI 1640 with 10% fetal calf serum.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2b,k6F185Affinity Purified
100ul-20CBlue IceHumanMouse
Protein S isolated from human plasma.
Purified by Protein A/G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Recognizes human Protein S. Reacts strongly with Protein S. Strong reaction is seen in sandwich ELISA in combination with a biotin-labeled antibody against protein S. Reactivity is Ca2+ dependent and is optimal when using Tris buffer containing 1mM calcium chloride. In Dot Blot, SDS-treatment abolishes the reactivity.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford. 2. Bendtzen K et al (2000) Basal og klinisk immunologi. FADLs Forlag A/S, Copenhagen.