Technical Data
Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human (PTP1B)
Molecular Biology Storage: -70CShipping: Dry Ice
Full-length (aa1-436) PTP1B with N-terminal GST-tag (GenBank Accession No. M31724)

Specific Activity:
~2,000 pmole/min/ug. One unit will hydrolyze 1nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30C

Assay Buffer:
50mM Hepes, pH 7.4, 2mM EDTA, 3mM DTT, 100mM sodium chloride, 50mM pNPP.

Suitable for use in enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Other applications not tested.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months.For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight:
Source: E. coli
Purity: 55 % by SDS-PAGE
Concentration: As reported
Form: Supplied as a liquid in 45mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4mM KCl, 10% glycerol, 18mM glutathione, and 3mM DTT

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Frangioni, et al., Cell 68: 545 (1992). 2. Elchebly, M., et al., Science 283: 1544 (1999).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.