Technical Data
P9109-01A
Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human (PTP1B)
20ug
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Technical Data |
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P9109-01A |
Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human (PTP1B) |
20ug |
| Molecular Biology | Storage: -70°CShipping: Dry Ice |
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Full-length (aa1-436) PTP1B with N-terminal GST-tag (GenBank Accession No. M31724) Specific Activity: ~2,000 pmole/min/ug. One unit will hydrolyze 1nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C Assay Buffer: 50mM Hepes, pH 7.4, 2mM EDTA, 3mM DTT, 100mM sodium chloride, 50mM pNPP. Applications: Suitable for use in enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Other applications not tested. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months.For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. CAS Number: N/A Molecular Weight: 76kD |
Source: E. coli Purity: 55 % by SDS-PAGE Concentration: As reported Form: Supplied as a liquid in 45mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4mM KCl, 10% glycerol, 18mM glutathione, and 3mM DTT Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Frangioni, et al., Cell 68: 545 (1992). 2. Elchebly, M., et al., Science 283: 1544 (1999).
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