Technical Data
P9109-14
Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)
250ug
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Technical Data |
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P9109-14 |
Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B) |
250ug |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. AA Sequence: MEMEKEFEQI/ DKSGSWAAIY/ QDIRHEASDF/ PCRVAKLPKN/ KNRNRYRDVS/ PFDHSRIKLH/ QEDNDYINAS/ LIKMEEAQRS/ YILTQGPLPN/ TCGHFWEMVW/ EQKSRGVVML/ NRVMEKGSLK/ CAQYWPQKEE/ KEMIFEDTNL/ KLTLISEDIK/ SYYTVRQLEL/ ENLTTQETRE/ ILHFHYTTWP/ DFGVPESPAS/ FLNFLFKVRE/ SGSLSPEHGP/ VVVHCSAGIG/ RSGTFCLADT/ CLLLMDKRKD/ PSSVDIKKVL/ LEMRKFRMGL/ IQTADQLRFS/ YLAVIEGAKF/ IMGDSSVQDQ/ WKELSHEDLE/ PPPEHIPPPP/ RPPKRILEPH/ N Specific Acitivity: 6,000-12,000units/mg. Unit Definition: One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate (p-NPP) per minute at pH 7.4, 37°C using 10mM of substrate. Storage and Stability: Store at -20°C or lower. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 37,312 |
Source: E. coli Purity: 95% by SDS PAGE; Purified by using conventional chromatography techniques. Concentration: ~1mg/ml Form: Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT, 20% glycerol. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Chernoff, J., et al., Proc. Natl. Acad. Sci. USA 87: 2735-2739 (1990).
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