Technical Data
Protein Tyrosine Phosphatase, STEP61/46 (Ser221/Ser49), Striatum-enriched protein-tyrosine phosphatase (STEP) isoform 61
Striatum-enriched protein-tyrosine phosphatase (STEP) isoforms 61 and 46 are two of the many splice variants for STEP, a protein belonging to the protein-tyrosine phosphatase family and containing a single tyrosine-protein phosphatase domain. STEP61 is membrane-associated, where as STEP46 is cytosolic. STEP61 also differs in that it contains a sequence of 172 amino acids with structural motifs not found in STEP46. Both isoforms are phosphorylated on serine residues, although STEP61 is mainly phosphorylated at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49. STEP is thought to have a critical role in regulating dopamine efficacy on striatial medium spiny neurons, neurotransmission through the NMDAR pathway, and AMPA receptor endocytosis.

Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1ug/ml detects STEP61/46 on 10ug of mouse brain tissue lysate.
Immunohistochemistry: 1:600 detects phosphorylated STEP61/46 in caudata putamen region of adult human brain tissue.
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse brain tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Synthetic peptide corresponding to mouse STEP61/46 phosphorylated at Ser221/Ser49 (KLH). Epitope: Phosphorylated Ser221/Ser49
Purified by affinity chromatography.
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.
Recognizes mouse STEP61. Species crossreactivity: human. Species sequence homology: rhesus monkey, bovine, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.