Technical Data
P9109-48E
6-Pyruvoyltetrahydropterin Synthase, PTS, Recombinant, Human (PTPS, PTP synthase)
100ug
Molecular Biology Storage: 4C/-20CShipping: Blue Ice
6-Pyruvoyltetrahydropterin synthase (PTS), also known as PTPS, belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia.

Recombinant human PTS, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Endotoxin: 1EU/1ug (LAL)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MSTEGGGRRC QAQVSRRISF SASHRLYSKF LSDEENLKLF GKCNNPNGHG HNYKVVVTVH GEIDPATGMV MNLADLKKYM EEAIMQPLDH KNLDMDVPYF ADVVSTTENV AVYIWDNLQK VLPVGVLYKV KVYETDNNIV VYKGE

Molecular Weight: 18.5kD (165aa)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight:
18.5kD
Source: E.coli
Purity: ~90% by SDS-PAGE, fused to His-tag at N-terminus.
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0,1mM DTT, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Thony B., et al. (1992) Biochem Biophys Res Commun. 189:1437-43. Milstien S., et al. (1989) J Biol Chem. 264(14):8066-73.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.