Technical Data
P9110-55
Proteoglycan, Cartilage, Adult
Description:
Proteoglycans are glycoproteins that are heavily glycosylated. They have a core protein with one or more covalently attached glycosaminoglycan (GAG) chain(s). The chains are long, linear carbohydrate polymers that are negatively charged under physiological conditions, due to the occurrence of sulfate and uronic acid groups. Proteoglycans occur in the connective tissue.

Applications:
Suitable for use in Western Blot, ELISA, RIA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:100-1:200
ELISA: 1:1250-1:2500
RIA: 1:20,000-1:40,000
Immunohistochemistry: 1:50-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q554Ascites
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human adult cartilage proteoglycan
Purity:
Ascites
Form
Supplied as a liquid.
Specificity:
Recognizes the short peptides substituted with keratan sulfate side chains and within the core protein of human proteoglycans in articular cartilages, chicken limb bud and cornea. Does not crossreact with human fetal cartilage proteoglycans. Species Crossreactivity: bovine, porcine, sheep, canine and chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Orthop. Trans. 9: 225-226 (1985). 2. J. Biochem. 234: 31-41 (1986). 3. J. Biochem. 236: 71-75 (1986). 4. Pediatric Res. 22: 409-413 (1987). 5. Cancer Immunol. Immunother. 32: 137-142 (1990). 6. J. Histochem. Cytochem. 39: 1175-1187 (1991). 7. Arthritis Rheum. 38: 660-668 (1995).