Technical Data
P9140
PRUNE (Prune homolog (Drosophila), DRES-17, HTCD37)
Description:
PRUNE, the human homologue of the Drosophila gene, is located in 1q21.3, a region highly amplified in human sarcomas, malignant tumors of mesenchymal origin. Human prune (h-prune), a phosphoesterase DHH family appertaining protein, physically interacts with nm23-H1, a metastasis suppressor gene. h-prune is involved in cellular motility and metastasis formation. Metastatic breast cancers were found to overexpress h-prune; h-prune was also found to be highly expressed in colorectal and pancreatic cancers. Hence h-prune is considered useful as a marker for tumor aggressiveness.

Applications:
Suitable for use in Immunocytochemistry Immunohistochemistry and Western Blot. Not suitable for Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 1:100
Immunohistochemistry (Frozen): 1:100
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM6D711Highly Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human PRUNE.
Purity:
Purified by affinity chromatography. 80-90% (SDS-PAGE).
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human PRUNE. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Citations: 1. Overexpression of h-prune in breast cancer is correlated with advanced disease status: M. Zollo, et al.; Clin. Cancer Res. 11, 199 (2005) GENERAL 1. Evidence for interaction between human PRUNE and nm23-H1 NDPKinase: A. Reymond, et al.; Oncogene 18, 7244 (1999). 2. Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas-a possible mechanism for altering the nm23-H1 activity: A. Forus, et al.; Oncogene 20, 6881 (2001). 3. Prune cAMP phosphodiesterase binds nm23-H1 and promotes cancer metastasis: A. D'Angelo, et al.; Cancer Cell 5, 137 (2004)