Technical Data
Media
Pseudomonas F Agar (Powder)
P9175
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation g/L:
Tryptone10.0
Dipotassium Phosphate 1.5
Proteose Peptone10.0
Magnesium Sulfate1.5
Agar15.0
total:38g/L
Enhancement of pyocyanin production of Pseudomonas.

Appearance:
Light beige, homogeneous, free flowing powder

Solubility:
Yellow beige, clear to trace hazy

pH: 7.0 0.2

Microbiological Analysis:
Note: Growth results obtained from type cultures after incubation at 37C and observed after 18-24 hours.

Pseudomonas aeruginosa
ATCC 10145
Pseudomonas aeruginosa
ATCC 2785

Components shown as g/L:

Tryptone 10.0g
Dipotassium Phosphate 1.5g
Proteose Peptone 10.0g
Magnesium Sulfate 1.5g
Agar 15.0g

Total: 38g/liter

Directions per Liter:
Dissolve 38g and 10g of glycerol in 800-900ml of dH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Directions per Liter: Dissolve 38 grams in 800-900ml of ddH2O containing 10g of glycerol, stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.