Technical Data
Media
Pseudomonas Isolation Broth (Powder)
P9175-20A
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation g/L:
Irgasan (R) 0.025
Magnesium Chloride1.4
Pancreatic Digest of Gelatin20.0
Potassium Sulfate10.0
Total:31.4g/L
Pseudomonas Isolation Broth is a modification of a formula described by King et al. Pseudomonas Isolation Broth was found useful in the isolation of Pseudomonas from pharmaceutical preparations (lotions and creams). The incorporation of lrgasan (R) inhibits most bacteria but allows the growth of Pseudomonas. The formulation also enhances pyocyanin production.


Directions per Liter: Dissolve 31.4 grams in 800-900ml of ddH2O containing 20ml of glycerol, stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Off-white to tan, homogeneous, free flowing powder

Solubility: Light yellow to tan, clear, complete with heat

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.