		Technical Data
P9182-75	PTTG 1 (Pituitary Tumor-Transforming Gene 1)

Description:
Pituitary tumor-transforming gene 1 (PTTG-1) is an oncogene that is expressed in normal tissues containing proliferating cells and in tumors. PTTG is downstream of the MAP kinase cascade and its phosphorylation at Ser162 by MAPK in vitro. The phosphorylation plays an essential role in PTTG’s transactivation function. PTTG has a DNA-binding domain, and it activates c-Myc (an oncogene) transcription by binding to the promoter in a protein complex. Studies found that expression of human PTTG correlates with cell proliferation in a cell cycle-dependent manner and the association of hPTTG with Ku, the regulatory subunit of DNA-dependent protein kinase, is disrupted when a DNA double-strand breaks occurs.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 2-4ug/ml. Recognizes only the 28kD band in MCF-7 cell lysate.
Immunohistochemistry: 1-2ug/ml in formalin-fixed, paraffin embedded tissues. For best results heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
I1904-47J: IgG, H&L (AP) Pab GtxRt
I1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide derived from the transactivation domain within the N-terminus of the human PTTG-1 protein

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes human PTTG-1. Species sequence homology: mouse PTTG-1. Reactivity confirmed with human MCF-7 cell lysate (Westerb Blot) and colon cancer tissue (IHC, paraffin).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Dominguez, A., et al., Oncogene, 17(17): 2187-2193 (1998). 2. Heaney, A.P., et al., Nat. Med., 5(11): 1317-1321 (1998). 3. Pei, L., et al., J. Biol. Chem., 276(11): 8484 (2000). 4. Pei, L., J. Biol. Chem., 275(40): 31,191-31,198 (2000 ). 5. Romero, F., et al., Nucleic Acids Res., 29(6): 1300-1307 (2001). 6. Zhang, X., et al., J. Clin. Endocrinol. Metab., 84(2): 761-767 (1999).