Technical Data
P9191-25
PUF60 (60kD Poly(U)-binding-splicing Factor, Fuse-binding Protein-interacting Repressor, FBP-interacting Repressor, Poly-U Binding Splicing Factor PUF60, Pyrimidine Tract-binding Splicing Factor, Ro Ribonucleoprotein-binding Protein 1, Ro-binding Protein
Description:
PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. PUF60 also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for PUF60 gene.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Cellular Localization: Cytoplasmic

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: YDQERFDNSDLSA, representing the C-terminus of the human protein (530-542), according to NP_055096; NP_510965.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Specificity:
Recognizes both reported human isoforms of PUF60. Expected to crossreact with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Orangutan (100% identity with immunogen) and porcine (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Liu J et al. The FBP interacting repressor targets TFIIH to inhibit activated transcription. Mol Cell 5:331-41 (2000).