Technical Data
P9198-01F
PUMA, alpha (p53 Upregulated Modulator of Apoptosis)
Description:
PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:30
Western Blot: 1:1000-5000
Immunoprecipitation: 1:50
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:250-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG6k335Supernatant
SizeStorageShippingSourceHost
100ul -20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues in the C-term of human PUMA.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15 sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human PUMA. Predicted to detect both isoforms based on sequence analysis. The isoform B is ~18kD and isoform A is ~24kD. Isoform B is primarily recognized on the western blot gel.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yu, J., Zhang, L., Hwang, P.M., Kinzler, K.W. and Vogelstein, B., 2001. Mol. Cell 7:673-682. 2. Nakano, K. and Vousden, K.H., 2001. Mol. Cell 7:683-694. 3. Han, J. et al. 2001. Proc. Natl. Acad. Sci. USA 98:11318-11323.