Technical Data
Pvu II
Molecular Biology Storage: -20CShipping: Blue Ice
5'-C A G^C T G-3'
3'-G T C^G A C-5'

Proteus vulgaris

Unit Definition:
One unit is defined as the amount of PvuI required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.


Dilution/Storage Buffer:
Supplied as a liquid in10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.

Supplied With:
R1625 Restriction Enzyme Buffer A, 10X
Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9

R1625-02 Restriction Enzyme Buffer C, 10X Supplied as a liquid in 10mM Tris-HCl pH 7.5 10mM Magnesium chloride, 50mM sodium chloride, 0.1mg/ml BSA Inhibition Temperature: 37C.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Stable for at least 12 months
Double Digests:
Restriction Enzyme Buffer A is provided to simplify buffer selection for double digests. 98% of Restriction Endonucleased work well in a 1x or 2x concentration of theRestriction Enzyme Buffer A.

Number of Recognition Sites:
Lambda: 15
M13mp18/19: 3 pBR322: 1
pUC18/19: 2 pUC57 : 2
pTZ19R/U: 2
X174: 0

Thermal Inactivation:
PvuI is inactivated by incubation at 80C for 20min.

Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 10-fold overdigestion (15u/ug lambda DNA x 1 hours) with PvuII (see Star Activity).

Ligation/Recutting Assay: After 10-fold overdigestion (5u/ug DNA x 2 hours) with PvuII, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.06uM. More than 95% of these can be recut.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of PvuI for 4 hours.

Star Activity: A large excess of enzyme (15u/ug DNA x 1 hours) may result in star activity. Star activity appears at 10-fold overdigestion (10 units x 1 hour).

Methylation Effect: Dam:, Dcm, CpG, EcoKI: never overlaps-no effect
EcoBI: may overlaps- not determined

Stability during Prolonged Incubation: A minimum of 0.2units of PvuI are required for complete digestion of 1ug of lambda DNA in 16 hours at 37C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.