Technical Data
P9542-50C
Pyrophosphatase, Inorganic, Recombinant, E.coli, aa1-176, His-tag (Cytosolic Inorganic Pyrophosphatase, Pyrophosphate PhosphohydrolaPse)
100ug
Molecular Biology Storage: -20°CShipping: Blue Ice
Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations.

Source:
Recombinant corresponding to aa1-176 of E.coli ppa, fused with His-tag at N-terminus, expressed in E.coli.

AA sequence:
MGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE
AAKAEIVASF ERAKNK

Molecular Weight:
~21.9kD (196aa), confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~21.9kD
Source: E.coli
Purity: ~95% (SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8, 1mM DTT, 50mM sodium chloride, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Harold FM., et al (1966) Bacteriol Rev 30(4):772–94. Carman GM., et al (2006) Trends Biochem. Sci. 31(12):694–9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.