Technical Data
R0006-74C
RAB3 (Ras-related Protein Rab-3A, RAB3A Member RAS Oncogene Family, Rab3a)
Description:
Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D (Schluter et al., 2004). These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters (Coppola et al., 2002). Specifically, Rab3s are thought to play a key role in presynaptic vesicle trafficking and priming (Schluter et al., 2006).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat cortex lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Peptide corresponding to amino acid residues from the N-terminal region of rat Rab 3 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug per ml BSA, 50% glycerol.
Specificity:
Recognizes rat Rab-3. Species sequence homology: human, mouse and xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schluter OM, Schmitz F, Jahn R, Rosenmund C, Sudhof TC (2004) A Complete Genetic Analysis of Neuronal Rab3 Function. J Neuroscience 24(29):6629-6637
2. Schluter OM, Basu J, Sudhof TC, Rosenmund C (2006) Rab3 Superprimes Synaptic Vesicles for Release: Implications for Short-term Synaptic Plasticity. J Neuroscience 26(4):1239-1246.
3. Coppola T, Perret-Menoud V, Gattesco S, Magnin S, Pombo I, Blank U, Regazzi R (2002) The death domain of Rab3 guanine nucleotide exchange protein in GDP/GTP exchange activity in living cells. J Biochem 362 (Pt.2):273-9.