Technical Data
R0007-14
B-Raf, Phosphorlyated (Thr401)
Description:
B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Applications:
Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunocytochemistry: 1:100
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9E509Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A phospho specific peptide corresponding to residues surrounding threonine 401 of human B-Raf.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human B-Raf phosphorylated on threonine 401. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hagemann C., Rapp U. R. Cell Res., 253: 34-46, 1999 2.Heidecker et al. 1990 Mol. Cell. Biol. 10:2503-2512. 3. Jaiswal, R. K.,et al. (1994) Mol. Cell. Biol. 14, 6944-6953 4.Brose et al. Cancer Res., 62: 6997-7000, 2002