Technical Data
R0009
Rab11A (RAB11A Member RAS Oncogene Family, Rab-11A, MGC1490, RAB11, Rab-11, Ras-related Protein Rab-11A, Ras-related protein Rab11A, YL8)
Description:
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25kD (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway. Rab11 is a member of the YPT-1 subfamily of Ras-related GTP-binding proteins. Rab11 mRNA has been detected in a variety of tissues including brain, testis, spleen, heart and gastrointestinal mucosa. In the gastric fundus the Rab11 protein is expressed in parietal cell tubulovesicles membranes. Expression of the Rab11 protein appears to occur in discrete epithelial cell populations where it localizes to apical vesicular populations.

Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Electron Microscopy. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
ELISA: 0.1-1ug/ml
Immunoprecipitation: 5ug
Immunofluorescence: 10-20ug/ml
Optimal dilutions to be determined by the researcher.

Recommended positive control:
Human HEK-293 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal hypervariable region of the human Rab11a sequence. Sequence Homology: Rab11b protein-10/17.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Rab11A protein at ~25kD. Species Crossreactivity: rat, rabbit and canine. Cross-reactivity with other Rab family members has not been detected.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Goldenring, J.R., et al., Am. J. Physiol. 270: G515-G525 (1996). 2. Goldenring, J.R., et al., Am. J. Physiol. 267: G187-G194 (1994). 3. Urbe, S., et al., FEBS Lett 334: 175-182 (1993). 4. Lai, F., et al., Genomics 22: 610-616 (1994). 5. Sheff, D.R., et al., J. Cell Biol. 145: 123-139 (1999). 6. Jou, T.S., et al., J. Cell Biol. 142(1): 85-100 (1998). 7. Chen, L., et al., J. Biol. Chem. 273: 33524-33532 (1998). 8. Chen, D., et al., Molecular Endocrinology 13: 993-1004 (1999). 9. Casanova, J.E., et al., Mol. Biol. Cell 10: 47-61 (1999).