Technical Data
R0009-03
Rab11A (RAB11A Member RAS Oncogene Family, Rab-11A, MGC1490, RAB11, Rab-11, Ras-related Protein Rab-11A, Ras-related protein Rab11A, YL8)
Description:
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25kD (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway. Rab11 is a member of the YPT-1 subfamily of Ras-related GTP-binding proteins. Rab11 mRNA has been detected in a variety of tissues including brain, testis, spleen, heart and gastrointestinal mucosa. In the gastric fundus the Rab11 protein is expressed in parietal cell tubulovesicles membranes. Expression of the Rab11 protein appears to occur in discrete epithelial cell populations where it localizes to apical vesicular populations.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 5ug/ml. Detects a ~22kD protein representing Rab 11 in CV1, PC3, and HeLa cell
extract. Another less prominent, unknown band is also detected by this antibody at ~35kD.
Optimal dilutions to be determined by the researcher.

positive control:
HEK293 cells

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-17, MGTRDDEYDYLFKVVLIC, from human Rab11.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human Rab11a protein at ~25kD. Species Crossreactivity: primate. Species Sequence homology: mouse and rat: 100%, C. elegans and amphibians: 94%. Cellular Localization: located on cytoplasmic face of organelles and vesicles.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Goldenring, J.R., et al., Am. J. Physiol. 270: G515-G525 (1996). 2. Goldenring, J.R., et al., Am. J. Physiol. 267: G187-G194 (1994). 3. Urbe, S., et al., FEBS Lett 334: 175-182 (1993). 4. Lai, F., et al., Genomics 22: 610-616 (1994). 5. Sheff, D.R., et al., J. Cell Biol. 145: 123-139 (1999). 6. Jou, T.S., et al., J. Cell Biol. 142(1): 85-100 (1998). 7. Chen, L., et al., J. Biol. Chem. 273: 33524-33532 (1998). 8. Chen, D., et al., Molecular Endocrinology 13: 993-1004 (1999). 9. Casanova, J.E., et al., Mol. Biol. Cell 10: 47-61 (1999).