Technical Data
R0009-82
RAB24, phosphorylated (Ser95) (Ras-related Protein Rab-24)
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The GTPase Rab24 is thought to be involved in the regulation of vesicular transport associated with autophagy.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser95 of human RAB24 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human RAB24 when phosphorylated at Ser95.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Maltese,W.A., BMC Cell Biol. 3, 25 (2002). 2.Erdman,R.A., J. Biol. Chem. 275(6), 3848-3856 (2000). 3.Olkkonen,V.M., J. Cell. Sci. 106(PT 4), 1249-1261 (1993).