		Technical Data
R0009-82A	RAB24, phosphorylated (Tyr17) (Ras-related Protein Rab-24)

Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The GTPase Rab24 is thought to be involved in the regulation of vesicular transport associated with autophagy.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr17 of human RAB24 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human RAB24 when phosphorylated at Tyr17.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Maltese,W.A., BMC Cell Biol. 3, 25 (2002). 2.Erdman,R.A., J. Biol. Chem. 275(6), 3848-3856 (2000). 3.Olkkonen,V.M., J. Cell. Sci. 106(PT 4), 1249-1261 (1993).