Technical Data
Rac1 (Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1), Cell Migration-inducing Gene 5 Protein, MGC111543, MIG5, p21-Rac1, Ras-like Protein TC25, Ras-related C3 Botulinum Toxin Substrate 1, TC25, TC-25)
Ras related C3 botulinum toxin substrate 1 (RAC1) protein is a GTPase which belongs to the RAS superfamily of small GTP binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases.

Suitable for use in Western Blot, Immunohistochemistry, Affinity Precipitation and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml. 20ug of rat brain microsomal prep was resolved by electrophoresis, transferred to PVDF or nitrocellulose and probed with R0400 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry: 10ug/ml. Gave positive immunostaining of rat brain sections fixed first with PBS, 4% paraformaldehyde, 2% acetic acid for 30 minutes followed by ethanol:acetic acid [95:5] for 2 minutes.
Affinity Precipitation Assay: 5-10ug of R0400-55A PAK-1 PBD agarose (as part of R0400-55 Rac1 Activation Assay Kit, BioAssay™) precipitated Rac1-GTP in lysates of 3T3 fibroblasts that were loaded in vitro with GTP-g-S. Proteins were detected by Western Blot anaylsis using 1ug/ml.
Immunoprecipitation: 4ug immunoprecipitated Rac1 from a rat brain microsomal protein preparation.
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2b0.T.127Affinity Purified
250ug-20°CBlue IceHumanMouse
Recombinant protein corresponding to the full length human Rac1 (Accession # NP_008839)
Purified by Protein G affinity chromatography.
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Recognizes human Rac1 at ~21kD. Slight Rac2 cross-reactivity is observed. An additional unknown protein of ~55kD may be detected in some preparations. Species Crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Gronborg, M. et al., (2006) Molecular and Cellular Proteomics, 5; 157-171. 1. Kjoller, L., and Hall, A. J. Cell Bio.. 152: 1145-1157 (2001). 2. Diakonova, M., et al., J. Biol. Chem. 277: 10,669-10,677 (2002). 3. Kovacic, H., et al., J. Biol. Chem. 276: 45,856-45,861 (2001). 4. Castoria, G., et al., J. Cell. Biol. 161: 547-556 (2003). 5. Gallager, B.C., et al., J. Cell. Sci. 116: 3239-3250 (2003). 6. Gismondi, M., et al., J. Immunol. 170: 3065-3073 (2003).