Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1-3ug/ml
Immunohistochemistry (Paraffin): 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Synthetic peptide corresponding to C-GRQGNFFASPMLK, from human RACGAP1, at C-terminal (NP_037409.2, NP_001119576.1, NP_001119575.1).|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.|
|Recognizes human RACGAP1. Species sequence homology: Bovine and canine.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Seguin L, Liot C, Mzali R, Harada R, Siret A, Nepveu A, Bertoglio J. CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase. Mol Cell Biol. 2009 Jan;29(2):570-81. 2. Grewal S, Carver JG, Ridley AJ, Mardon HJ. Implantation of the human embryo requires Rac1-dependent endometrial stromal cell migration. Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16189-94. 3. Touré A, Mzali R, Liot C, Seguin L, Morin L, Crouin C, Chen-Yang I, Tsay YG, Dorseuil O, Gacon G, Bertoglio J. Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase. FEBS Lett. 2008 Apr 9;582(8):1182-8. 4. Zhao WM, Seki A, Fang G. Cep55, a microtubule-bundling protein, associateswith centralspindlin to control the midbody integrity and cell abscission during cytokinesis. Mol Biol Cell. 2006 Sep;17(9):3881-96. 5. Nishimura Y, Yonemura S. Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis. J Cell Sci. 2006 Jan 1;119(Pt 1):104-14. Epub 2005 Dec 13.|