Technical Data
R0451-01BX
Rad51 (BRCC5, DNA Repair Protein RAD51 Homolog 1, E. coli RecA Homolog, HGNC:9817, HRAD51, HsRad51, HsT16930, RAD51, RAD51A, RecA Homolog (E. coli), RecA-like Protein) (BSA & Azide Free)
Description:
Rad51 (a eukaryotic homologue of Escherichia coli RecA) is similar to RecA, both biochemically and structurally: It promotes homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57 and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C and RAD 51D). Recent studies suggested that Rad51 is involved not only in homologous recombination but also in cell proliferation regulation.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation (Native verified): 2ug/mg protein lysate. Use Protein G.
Western Blot: 1-2ug/ml for 2 hrs at RT
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells and testis.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F326Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant Rad51 protein. Cellular Localization: Nuclear. MW of Antigen: 37-43kD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See R0451-01B.
Specificity:
Recognizes human Rad51. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Barlow, A.L., et al., EMBO J. 16(17): 5207-5215 (1997).