Technical Data
R0495-55
RKIP (Raf kinase inhibitor protein) (Raf kinase inhibitor protein, phosphatidylethanolamine binding protein 1, PEBP)
Description:
Raf kinase inhibitory protein (RKIP) belongs to the phosphatidylethanolamine-binding protein (PEBP) family. It is an inhibitor of the Raf/MEK/MAP kinase signaling cascade and is a suppressor of cancer metastasis (1). RKIP is involved in neural development, cardiac function and spermatogenesis and appears to have serine protease activity. The inhibition Raf-1-mediated phosphorylation of MEK results from RKIP binding to Raf-1. Dysregulated RKIP expression has the potential to contribute to pathophysiological processes including Alzheimer's disease and diabetic nephropathy (2). RKIP represents a novel effector of signal transduction pathways leading to apoptosis and a prognostic marker of the pathogenesis of human cancer cells and tumors (3).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:100
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E514Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues in human RKIP.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human RKIP (Raf kinase inhibitor protein). Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Eves EM, et al. Mol Cell. 23(4):561-74, 2006. 2. Keller E T, et al. Biochemical pharmacology 68(6):1049-1053, 2004. 3. Chatterjee D, et al. J Biol Chem. 279(17):17515-23, 2004